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efluor 506 fixable viability dye  (Thermo Fisher)


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    Structured Review

    Thermo Fisher efluor 506 fixable viability dye
    Efluor 506 Fixable Viability Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fixable+viability+dye+efluor+506/PBS/pmc12749970-518-7-12
    Average 98 stars, based on 1 article reviews
    efluor 506 fixable viability dye - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: In Vitro Modeling of Natural Killer Cell Cytotoxicity to Inform Personalized ALS Therapeutics.
    Article Snippet: .. Following collection and wash, NK and K- 562 co- cultures were incubated with Fixable Viability Dye eFluor 506 (1:500) (eBioscience, cat #65–0866- 14) during the blocking stage. .. Samples were then incubated with the surface stains CD56 (Biolegend, cat #318318; RRID:AB_604107), CD11a (Biolegend, cat #301207; RRID:AB_10660819), and CD71 (Biolegend, cat #334110; RRID:AB_2563117) at 4°C for 30 min. K- 562 cells were identified as CD56- , CD11a- , and CD71+.

    Blocking Assay:

    Article Title: In Vitro Modeling of Natural Killer Cell Cytotoxicity to Inform Personalized ALS Therapeutics.
    Article Snippet: .. Following collection and wash, NK and K- 562 co- cultures were incubated with Fixable Viability Dye eFluor 506 (1:500) (eBioscience, cat #65–0866- 14) during the blocking stage. .. Samples were then incubated with the surface stains CD56 (Biolegend, cat #318318; RRID:AB_604107), CD11a (Biolegend, cat #301207; RRID:AB_10660819), and CD71 (Biolegend, cat #334110; RRID:AB_2563117) at 4°C for 30 min. K- 562 cells were identified as CD56- , CD11a- , and CD71+.

    Labeling:

    Article Title:
    Article Snippet: Frozen sternal BM aspiration and PBMCs were thawed at 37°C and then resuspended in RPMI 1640 (Life Technologies) supplemented with 10% FBS (Life Technologies), Penicillin-Streptomycin (Dutscher), L-glutamine (Life Technologies), Sodium Pyruvate (EuroBio), HEPES (EuroBio). .. Cells were treated for 15 min with 1000 U/ml of DNAse I. PBMCs and BM cells were first labeled in PBS 30min at 4°C with the fixable viability dye eFluor 506 (eBioscience), and then were stained in the same conditions in presence of brilliant stain buffer (Becton Dickinson Biosciences) with specific antibodies of surface markers. ..

    Staining:

    Article Title:
    Article Snippet: Frozen sternal BM aspiration and PBMCs were thawed at 37°C and then resuspended in RPMI 1640 (Life Technologies) supplemented with 10% FBS (Life Technologies), Penicillin-Streptomycin (Dutscher), L-glutamine (Life Technologies), Sodium Pyruvate (EuroBio), HEPES (EuroBio). .. Cells were treated for 15 min with 1000 U/ml of DNAse I. PBMCs and BM cells were first labeled in PBS 30min at 4°C with the fixable viability dye eFluor 506 (eBioscience), and then were stained in the same conditions in presence of brilliant stain buffer (Becton Dickinson Biosciences) with specific antibodies of surface markers. ..

    Article Title: Effects of Maackia amurensis seed lectin (MASL) on OSCC cell morphology, PDPN expression, growth, and motility in a phase 1 clinical trial
    Article Snippet: .. NIR light (150 mW/cm 2 ) was applied to achieve 0, 5, 10, 20, or 50 Joules (J) before cells were immediately trypsinized, collected, stained with Fixable Viability Dye eFluor 506 (ThermoFisher Scientific 65–0866), and analyzed by flow cytometry (FACSLyric, BD Biosciences, San Jose, CA, USA) with FlowJo software (BD Biosciences). .. Statistical analysis was performed with Prism software (GraphPad Prism version 10) as described in text and figure legends.

    Article Title: Controlled Release of Poly(U) via Acetalated Dextran Microparticles for Enhanced Vaccine Adjuvant Delivery
    Article Snippet: .. Splenocytes and inguinal lymph node lymphocytes were stimulated for 36hr as above and stained for flow cytometry using the following panel: eBioscience Fixable Viability Dye eFluor 506 (diluted 1:1000) and the following fluorescent antibodies from Biolegend (San Diego, CA): CD3 Alexa Fluor 488 (diluted 1:100), CD4 APC/Fire 750 (diluted 1:200), CD8 PerCP/Cy5.5 (diluted 1:100), CD44 Brilliant Violet 421 (diluted 1:400), CD62L Brilliant Violet 785 (diluted 1:400), CD19 APC (diluted 1:400), GL7 PE (diluted 1:1600), and CD38 PE/Cyanine7 (diluted 1:800) (Fig S1, Fig S2) . ..

    Flow Cytometry:

    Article Title: Effects of Maackia amurensis seed lectin (MASL) on OSCC cell morphology, PDPN expression, growth, and motility in a phase 1 clinical trial
    Article Snippet: .. NIR light (150 mW/cm 2 ) was applied to achieve 0, 5, 10, 20, or 50 Joules (J) before cells were immediately trypsinized, collected, stained with Fixable Viability Dye eFluor 506 (ThermoFisher Scientific 65–0866), and analyzed by flow cytometry (FACSLyric, BD Biosciences, San Jose, CA, USA) with FlowJo software (BD Biosciences). .. Statistical analysis was performed with Prism software (GraphPad Prism version 10) as described in text and figure legends.

    Article Title: Controlled Release of Poly(U) via Acetalated Dextran Microparticles for Enhanced Vaccine Adjuvant Delivery
    Article Snippet: .. Splenocytes and inguinal lymph node lymphocytes were stimulated for 36hr as above and stained for flow cytometry using the following panel: eBioscience Fixable Viability Dye eFluor 506 (diluted 1:1000) and the following fluorescent antibodies from Biolegend (San Diego, CA): CD3 Alexa Fluor 488 (diluted 1:100), CD4 APC/Fire 750 (diluted 1:200), CD8 PerCP/Cy5.5 (diluted 1:100), CD44 Brilliant Violet 421 (diluted 1:400), CD62L Brilliant Violet 785 (diluted 1:400), CD19 APC (diluted 1:400), GL7 PE (diluted 1:1600), and CD38 PE/Cyanine7 (diluted 1:800) (Fig S1, Fig S2) . ..

    Software:

    Article Title: Effects of Maackia amurensis seed lectin (MASL) on OSCC cell morphology, PDPN expression, growth, and motility in a phase 1 clinical trial
    Article Snippet: .. NIR light (150 mW/cm 2 ) was applied to achieve 0, 5, 10, 20, or 50 Joules (J) before cells were immediately trypsinized, collected, stained with Fixable Viability Dye eFluor 506 (ThermoFisher Scientific 65–0866), and analyzed by flow cytometry (FACSLyric, BD Biosciences, San Jose, CA, USA) with FlowJo software (BD Biosciences). .. Statistical analysis was performed with Prism software (GraphPad Prism version 10) as described in text and figure legends.

    other:

    Article Title: Serotonin transporter inhibits antitumor immunity through regulating the intratumoral serotonin axis.
    Article Snippet: OVA dextramer (catalog no. JD2163) was purchased from Immudex.



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